Postdoc Position in Gene Regulation

Cell identity relies on correct gene expression, so the process of transcription is tightly regulated. During early elongation of RNA Polymerase II, it is likely to terminate. Early termination has a big impact on transcriptional output, but how it is regulated is poorly understood. The Vlaming lab aims to understand what drives early termination vs productive elongation. To this end, we use cutting‑edge nascent‑RNA sequencing techniques, high‑throughput screening and precise genome editing.

This project aims to systematically identify and characterize regulators of early transcription termination. Potential approaches will involve pooled CRISPR screens, genome editing mouse embryonic stem cells to allow for rapid protein depletion, mapping transcriptional effects (RNA‑seq, TT‑seq, PRO‑seq), proximity labeling approaches, ChIP, and the bioinformatic analysis of generated sequencing data. You are encouraged to bring your own expertise to the current project and contribute your own project ideas.

While working on this project, you will hone your technical skills as well as skills in project design, writing, presenting, supervising, and collaborating. As part of our team, you will contribute to an enthusiastic, collaborative and respectful environment, in which we can perform high‑quality research together.