PhD position in molecular mechanisms of bleeding disorders NWO-funded project on the molecular and cellular mechanisms of secretory organelle biogenesis and exocytosis in bleeding disorders.

• Closing date 01-05-2024
• 16.13.24.TDdH

• 36 hours
• Research university
• Research & Education

• Closing date 01-05-2024
• 16.13.24.TDdH

Job description Job description

Von Willebrand Factor (VWF) plays a pivotal role in blood coagulation. VWF is secreted from endothelial cell Weibel-Palade bodies (WPBs), which is crucial for the response to vascular trauma. Dysfunctional WPB biogenesis and exocytosis are characteristic of bleeding disorders such as Von Willebrand disease. In this project, we aim to identify the organelle trafficking routes responsible for WPB formation and secretion.

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Two PhD positions are available, one at each institute.

The Erasmus MC candidate will conduct biochemical assays of WPB cargo secretion and optical assays of WPB exocytosis to determine changes in secretory competence and fusion mode due to altered SNARE sorting. Techniques include high content microscopy, proteomics, total internal reflection fluorescence (TIRF) microscopy, shRNA library screenings, and electron microscopy.

The UG candidate will utilize newly developed microscopy methods, including Förster resonance energy transfer-fluorescence lifetime imaging (FRET-FLIM), to map SNARE protein trafficking to WPBs. Techniques include FRET-FLIM microscopy, mammalian cell culturing, CRISPR, and molecular cloning.

The PhD candidates are expected to also spend research time at the other institute.

https://www.hematologyrotterdam.nl/laboratory-research/ruben-bierings) we use in vitro and ex vivo experimental studies on (patient-derived) endothelial cells with the objective to elucidate the mechanisms that regulate secretion of hemostatic proteins, in health and disease. 

Qualifications and skills

If you are ready to tackle the molecular cell biology of bleeding disorders, we encourage you to apply for this exciting PhD opportunity at Erasmus MC. You have the following qualifications:

• A university MSc degree in molecular biology, biochemistry, cell biology, biomedical sciences or any other field that has affinity with basic and/or translational research.
• Excellent communication and presentation skills. Fluency in English (oral and written) is a prerequisite.
• Self-motivated, detail-oriented, and adept at both independent and collaborative work.
• Extensive hands-on practical laboratory experience is required, previous experience with mammalian cell biology and advanced microscopy approaches is an advantage.
• Willing to spend research time in Groningen.

Before you apply please check our conditions for employment.

Terms of employment Terms of employment

• You will receive a temporary position for 4 years. The gross monthly salary is € 2.901,- in the 1st year and increases to € 3.677,- in the 4th year (scale OIO).
• Excellent fringe benefits, such as a 13th month that is already paid out in November and a individual travel expense package.
• Pension insurance with ABP. We take care of approximately 2/3 of the monthly contribution.
• Special benefits, such as a incompany physiotherapist and bicycle repairer. And there is also a gym where you can work on your fitness after work.

 
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• Motivation letter (max 1 page).
• Up-to-date CV (max 2 pages).
• List of grades for MSc degree.
• Contact details or letters of recommendation of two references.

Screening of applications will start as soon as applications are received and will continue until the position is filled.

No agencies please.